ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Developing pancreases from embryos of thirteen and one-half to seventeen and one-half days were placed on rayon grids and cultivated on a liquid medium of cock serum and chick embryo extract for periods of two to ten days. The cultures were incubated at 37°C in air supplemented by controlled oxygen and carbon dioxide.Pancreatic islets develop well in organ culture. This process usually involves increases in the size of islets and in the number of cells per islet, and sometimes involves an increase in the number of islets. The rate of morphogenesis of islets is variable: slower than that in normal controls during equal time in days (most cases) or equal to it (several cases) or faster than it (few cases). The development of islets includes the formation of granulated beta cells (many cases). The beta cells may reach stages as advanced as those in normal pancreases of postnatal day 3 (few cases). The existence of granulated beta cells in cultures from explants too young to have them constitutes convincing morphologic evidence of the production of insulin or pre-insulin during the periods of culture.The development of acini in organ culture includes the formation of them from pancreatic primordia with no acini and the appearance of zymogen granules in the acinar cytoplasm. The morphogenesis may reach stages as advanced as those in normal pancreases of prenatal day nineteen and one-half (several cases).
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091470111
