ISSN:
0021-9541
Schlagwort(e):
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Medizin
Notizen:
A recently described pituitary chondrocyte growth factor (CGF) is a contaminant of several related glycoprotein hormones (TSH, LH and HCG). Chondrocytes were cultured from rabbits two to three months old. Bovine TSH (NIH) 69.5 μg/ml, used as the source of CGF, reduced the generation time from 16 to 10 hours through a virtual effacement of the G1 period. Incorporation of 3H-thymidine declined rapidly after 48 hours from maximal values (control 300 cpm/μg DNA; CGF, 679). Total DNA accumulated thereafter until 116 hours when the figures were 36 and 98 μg/flask, respectively. Little growth response occurred in spinner cultures. CGF lowered plating efficiency from 4.5 to 2.3%. The stimulatory effect diminished when CGF was removed from the medium. The treated cells were smaller and contained less protein and RNA than controls. They synthesized smaller quantitites of sulfated mucopolysaccharides (chondroitin sulfates 4,6 and doubly sulfated chondroitin as well as some dermatan sulfate) but hyaluronate production was not diminished.
Zusätzliches Material:
2 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jcp.1040840203