ISSN:
1420-908X
Keywords:
Key words: MCP-1 – Macrophage infiltration – Neutrophil infiltration – IL-8 – LPS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Objective and Design: To evaluate the mechanism whereby monocyte chemoattractant protein (MCP)-1 attracts monocytes in vivo.¶Subjects: New Zealand white rabbits (175 rabbits) were used.¶Treatment: LPS, MCP-1 or IL-8 was injected into knee joints. Antibodies against various cytokines or IL-1 receptor antagonist were injected to neutralize cytokine activities.¶Methods: The numbers of leukocyte populations, levels of cytokines in joints were estimated.¶Results: Partial inhibition of neutrophil influx with anti-IL-8 IgG (10 μg) suppressed LPS-induced macrophage influx by 43 ± 8.5% (p〈0.05) without affecting the MCP-1 level. Intraarticular injection of MCP-1 (1-30 μg) induced macrophage influx. The event was accompanied by a small num-ber of neutrophils in an early phase. Co-injection of IL-8 (1.0 μg) enhanced the MCP-1-induced macrophage infiltration (p〈0.01). In neutrophil-deplete d rabbits, LPS failed to induce macrophage influx even though the MCP-1 level was maintained, and macrophage influx following exogenously administered MCP-1 was also dramatically inhibited.¶Conclusions: Early events associated with neutrophil infiltration appear to be important for MCP-1 to induce a later macrophage influx in LPS-arthritis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s000110050645
