ISSN:
1572-9699
Keywords:
Agglutination
;
Mating
;
Yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The agglutinability-masking factors (AMFs) of a and α mating types of Issatchenkia scutulata var. scutulata were prepared from culture fluids. AMFs masked the agglutinability of opposite mating-type cells sex-specifically, just like agglutination substances responsible for sexual cell agglutination. a AMF adsorbed to α cells was eluted by incubating the cells at 60°C for 10 min. α AMF was prepared directly from culture fluids of α cells by DEAE-cellulose ion exchange chromatography. The active part of the AMFs is thought to be a peptidyl moiety because of the sensitivity to subtilisin. The pretreatment of cells with AMF of the opposite mating-type was shown to promote zygote formation. α AMF slightly inhibited growth in a cells but not in α cells, while a AMF did not show any growth-inhibitory effect on either a or x cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00393464
