ISSN:
1573-5028
Keywords:
GT-1
;
induced expression
;
PR proteins
;
salicylic acid
;
trans-acting factors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The 900 by promoter region of the tobacco PR-1a gene was divided into eight fragments using PCR. The fragments were tested for their ability to bind to nuclear factors isolated from tobacco leaf. Band shift assays demonstrated that all but one of the fragments specifically interacted with nuclear proteins. From competition experiments it was determined that the same nuclear factors bind various promoter fragments with different affinity. Moreover, efficient competition with a synthetic tetramer of box II of the rbcS promoter (Green PJ et al., EMBO J 13 (1988) 4035–4044) indicated that GT-1-like nuclear factors are involved in these interactions. Furthermore, in comparison to extracts from untreated plants, nuclear protein preparations from tobacco mosaic virus-infected tobacco showed a reduced GT-1 binding activity. These results will be discussed in relation to induced PR-1a gene expression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00049327
