ISSN:
1573-5044
Keywords:
vinca major
;
cell suspension
;
culture
;
chlorophyll content
;
indole alkaloids
;
strictosidine lactam
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two cell lines of Vinca major L. cv. variegata produced strictosidine lactam as the main alkaloid. Quantities varied from 500–1000 μg/g DW in the high-yielding line and 1–100 μg/g DW in the low-yielding line. Transfer of cells to alkaloid production medium resulted in a 6–8 fold increase in alkaloid production with the high-yielding line, some increase in the low-yielding line and in both lines induced intense greening (up to 200 μg chlorophyll/g DW) indicating chloroplast differentiation. Though in the source plant leaves are the main storage site, no correlation between alkaloid accumulation and chloroplast differentiation could be found.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043154