ISSN:
1573-4935
Keywords:
phosphoinositide hydrolysis
;
phospholipase C
;
cell proliferation
;
lectins
;
lymphocytes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%) of PHA (10 μg/ml) or ConA (25 μg/ml) proliferative effects. Thus, the activation of membrane-bound PLC is asine-qua-non condition for lectin-induced proliferation of T lymphocytes. The key-role of PLC versus protein kinase C (PKC) is stressed by the fact that the inhibition of PKC with Hidaka's compound H-7 (40 μM) produced only a partial blockade (about 25%) of lectin mitogenic effect.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01116866
