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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 44 (1966), S. 983-984 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The maximum elimination of leucocytes and thrombocytes is a presupposition for the performance of metabolic studies on red cells. Depending on the experimental purpose an elimination rate up to 1–0,1 leucocytes resp. 〈50 thrombocytes per 104 red cells is necessary. Centrifugation-and washing-methods, mostly used nowadays for separation of erythrocytes, do not allow such a far-reaching isolation. The usage of cotton wool, however, introduced byFleming in 1926, can be done quickly and easily and solves this experimental problem.
    Notes: Zusammenfassung Für Stoffwechseluntersuchungen an Erythrocyten ist die weitestgehende Elimination der Leuko-und Thrombocyten Voraussetzung, und zwar in Abhängigkeit vom Versuchsziel bis auf 1–0,1 Leuko- bzw. 50 Thrombocyten/104 Erythrocyten. Eine derartige Isolierung ist mittels der gebräuchlichen Zentrifugations- und „Wasch“-Verfahren der roten Zellen ohne Reticulocytenverlust unmöglich. Sie gelingt hingegen unter Verwendung von Baumwolle (inauguriert vonFleming, 1926) rasch und mühelos.
    Type of Medium: Electronic Resource
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