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  • 1
    Electronic Resource
    Electronic Resource
    Erythrocytenisolierung aus Blut mit Baumwolle (1966)
    Springer
    Journal of molecular medicine 44 (1966), S. 983-984 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The maximum elimination of leucocytes and thrombocytes is a presupposition for the performance of metabolic studies on red cells. Depending on the experimental purpose an elimination rate up to 1–0,1 leucocytes resp. 〈50 thrombocytes per 104 red cells is necessary. Centrifugation-and washing-methods, mostly used nowadays for separation of erythrocytes, do not allow such a far-reaching isolation. The usage of cotton wool, however, introduced byFleming in 1926, can be done quickly and easily and solves this experimental problem.
    Notes: Zusammenfassung Für Stoffwechseluntersuchungen an Erythrocyten ist die weitestgehende Elimination der Leuko-und Thrombocyten Voraussetzung, und zwar in Abhängigkeit vom Versuchsziel bis auf 1–0,1 Leuko- bzw. 50 Thrombocyten/104 Erythrocyten. Eine derartige Isolierung ist mittels der gebräuchlichen Zentrifugations- und „Wasch“-Verfahren der roten Zellen ohne Reticulocytenverlust unmöglich. Sie gelingt hingegen unter Verwendung von Baumwolle (inauguriert vonFleming, 1926) rasch und mühelos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01711475
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  • 2
    Electronic Resource
    Electronic Resource
    Nosocomial Infections in a Neurosurgery Intensive Care Unit (1999)
    Springer
    Acta neurochirurgica 141 (1999), S. 1303-1308 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Intensive care unit; neurosurgery; nosocomial infection; surveillance.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary ¶ In order to identify overall and site-specific nosocomial infection (NI) rates in patients receiving neurosurgical intensive care therapy, a prospective study was started in February 1997 in the eight-bed neurosurgical ICU of the University Hospital of Freiburg, Germany. Case records were reviewed twice a week, all microbiology reports were reviewed and ward staff was consulted. NI were defined according to the CDC-criteria and were categorised into specific infection sites. Within 20 months, 545 patients with a total of 5,117 patient days were investigated (mean length of stay: 9.4 days). 113 NI were identified in 90 patients (72 pts. with one, 13 with two and 5 with three infections, respectively). A moderate to high overall incidence (20.7/100 pts.) and a moderate incidence density (22.1/1,000 patient days) of NI in the neurosurgical ICU could be documented; these figures are well within the range of published data. Site specific incidence rates and incidence densities were: 1 bloodstream infection per 100 patients (0.9 central line-associated BSIs per 1,000 central line-days), 9 pneumonias per 100 patients (15.1 ventilator-associated pneumonias per 1,000 ventilator-days), 7.3 urinary tract infections per 100 patients (8.5 urinary catheter-associated UTIs per 1,000 urinary catheter-days). Additionally, 1.1 cases of meningitis, 0.7 brain abscesses/ventriculitis, and 1.7 other infections (surgical site infection, bronchitis, catheter related local infection, diarrhoea) were documented per 100 patients, respectively. 14.6% of isolated pathogens were E. coli, 10.2% enterococci, 9.6% S. aureus, 6.4% CNS, 6.4% Klebsiella spp., 5% Enterobacter spp. and 5% Pseudomonas spp.. In 11 cases of NI no pathogen could be isolated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050434
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  • 3
    Electronic Resource
    Electronic Resource
    Prostaglandin IX - synthesis of (+/-)-15-methyl-11-deoxy PGE"1 (doxaprost) - a potent bronchodilator - and its C-15-epimer
    Amsterdam : Elsevier
    Prostaglandins 11 (1976), S. 981-986 
    Details
    ISSN: 0090-6980
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0090-6980(76)90006-X
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  • 4
    Electronic Resource
    Electronic Resource
    The programming language 'PEARL' and its implementation
    Amsterdam : Elsevier
    Computer Physics Communications 15 (1978), S. 317-324 
    Details
    ISSN: 0010-4655
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Computer Science , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0010-4655(78)90060-7
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  • 5
    Electronic Resource
    Electronic Resource
    Discrimination of Helicobacter pullorum and Campylobacter lari by analysis of whole cell fatty acid extracts (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 168 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Cholesteryl glucoside, a characteristic feature of the genus Helicobacter, but seldom found in other bacteria, could not be detected in Helicobacter pullorum. Therefore, rapid determination of this glycolipid may serve as a discrimination marker for Helicobacter pullorum from most other Helicobacter species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13275.x
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  • 6
    Electronic Resource
    Electronic Resource
    Fluorescence in situ hybridization for direct visualization of Gram-negative anaerobes in subgingival plaque samples (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 6 (1993), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1993.tb00312.x
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  • 7
    Electronic Resource
    Electronic Resource
    Lack of penetration of cephacetril into the cerebro-spinal fluid of patients without meningitis (1979)
    Springer
    Infection 7 (1979), S. 41-44 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Sechs neurochirurgische Patienten erhielten 2–4 g Cephacetril intravenös in sechsstündlichem Abstand. Serum und Liquorspiegel wurden bestimmt. Bei intakter Blut-Liquorschranke waren keine Cephacetrilkonzentrationen im Liquor nachweisbar. Bei zwei Patienten mit erhöhten Zellzahlen im Liquor wurde Cephacetril in niedrigen Konzentrationen (0,1–0,8 mcg/ml) nachgewiesen. Die Untersuchung zeigt, daß Cephacetril nur bei Entzündungen oder anderen Störungen der Blut-Liquorschranke in den Liquor gelangt. Die Ergebnisse einiger Autoren, die therapeutisch wirksame Liquorkonzentrationen von Cephacetril bei gesunden Patienten fanden, konnten wir nicht bestätigen.
    Notes: Summary Six neurosurgical patients were given 2–4 g cephacetril intravenously at six hourly intervals. Serum and cerebrospinal fluid (CSF) concentrations were determined. Cephacetril activity was not detectable in the CSF when the blood-CSF barrier was intact. In two patients with increased CSF cell counts cephacetril was found in the CSF in low concentrations (0.1–0.8 mcg/ml). Our investigations show that cephacetril does not penetrate into the CSF unless there is an inflammation or other impairment of the blood-CSF barrier. Thus we could not confirm the results of some authors who found cephacetril in therapeutically effective concentrations in the CSF of healthy persons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01640557
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  • 8
    Electronic Resource
    Electronic Resource
    Die Aminkolpitis — nicht nur ein ästhetisches Problem (1985)
    Springer
    Archives of gynecology and obstetrics 238 (1985), S. 836-837 
    Details
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02430230
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  • 9
    Electronic Resource
    Electronic Resource
    Measurements ofE4 matrix elements in152Sm and154Sm (1973)
    Springer
    The European physical journal 263 (1973), S. 191-200 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract TheE4 transition matrix element from the ground state to the 4+ state has been measured in152Sm and154Sm, by comparing relative thick targetγ-ray intensities following Coulomb excitation with 12 MeV4He, 24–30 MeV16O, and 35–40 MeV32S projectiles. Values for 〈0+∥ℳ(E4)∥4+〉 of (+0.31 −0.20 +0.12 ) barn in152Sm and (+0.50 −0.12 +0.09 ) barn in154Sm are found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01392561
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  • 10
    Electronic Resource
    Electronic Resource
    Klinische Prüfung der Effektivität und Verträglichkeit von Norfloxacin in der Therapie komplizierter Harnwegsinfektionen und in der Langzeitprophylaxe rezidivierender Harnwegsinfektionen (1987)
    Springer
    Infection 15 (1987), S. 20-24 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The efficacy and tolerance of norfloxacin in the treatment of complicated urinary tract infections and in low dose long-term prophylaxis over six months for recurrent UTI were investigated in 60 and 27 patients, respectively. In the prophylaxis group, compliance with medication was controlled weekly using Mecur®BT. Five to nine days after treatment, the urine was sterile in 50 out of the 60 patients treated (83.3%); relapse occurred in six patients and was associated with development of resistance in two. Four patients suffered re-infection within the study period. Failure of treatment was closely related to persisting complicating factors in the urinary tract. The overall tolerance of norfloxacin was satisfactory. However, there was one severe allergic reaction. During the total 391 weeks of low dose long-term prophylaxis given to 27 patients, only one break-through infection occurred. Yet compliance studies proved that antimicrobial activity was present in the urines of less than 50% of the patients. During prophylaxis, the rate of infection was reduced from three to four to 0.13 to 0.05 infections/patient/year. There was no development of resistance in the isolates from these patients. Only two adverse reactions were observed.
    Notes: Zusammenfassung Es wurde die Effektivität und Verträglichkeit von Norfloxacin in der Therapie komplizierter Harnwegsinfektionen bei 60 urologischen Patienten und in der Langzeit-Rezidivprophylaxe mit Niedrigdosen Über sechs Monate bei 27 Patienten untersucht. Fünf bis neun Tage nach Therapie bestand Keimfreiheit bei 50 der 60 Patienten (83,3%); bei sechs Patienten kam es zum Rezidiv, in zwei Fällen trat dabei eine Resistenzentwicklung auf. Bei vier Patienten wurde eine Reinfektion nachgewiesen. Therapieversager waren mit der Persistenz komplizierender Faktoren korreliert. Die Verträglichkeit war mit Ausnahme einer schweren allergischen Reaktion gut. In insgesamt 391 Wochen von Langzeitprophylaxe trat bei einem von 27 Patienten eine Durchbruchinfektion auf. Die einmal wöchentlich durch Micur®-BT kontrollierte Einnahmesicherheit hatte dabei unter 50% gelegen, so daß sich durch die Effektivität der Norfloxacin-Langzeitprophylaxe die Zahl der Durchbruchinfektionen von drei bis vier pro Patient pro Jahr auf 0,13 bis 0,05 reduzierte, dies bei zwei allergischen Reaktionen und ohne daß Resistenzentwicklung auftrat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01646113
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