ISSN:
1432-1440
Schlagwort(e):
Abbreviations ALL Acute lymphoblastic leukemia
;
BMT Bone marrow transplantation
;
CTL Cytotoxic T-lymphocytes
;
IL Interleukin
;
MHC Major histocompatibility
;
MRD Minimal residual diseaseIntroductionObjective
;
The purpose of this protocol is to induce a specific cellular immune reaction after autologous bone marrow transplantation (BMT) by vaccination with human leukocyte antigen (HLA) B13 transfected autologous leukemic cells in children with high-risk relapse of acute lymphoblastic leukemia (ALL). Specific cytotoxic T-lymphocytes (CTL) will be expanded in vivo by low-dose interleukin-2 (IL-2). The expression vector used is the pcDNA3 HLA-B13 plasmid
;
driven by a cytomegalovirus promoter. Leukemic cells are transfected by electroporation/ballistomagnetic transfer.
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s001090050211