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  • 1
    Electronic Resource
    Electronic Resource
    Improved in vitro selection of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 299-304 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana plumbaginifolia ; Mutant selection ; Nitrate reductase ; Protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The use of increasing knowledge on regulation of nitrate reductase activity in Nicotiana cell cultures is the basis for the described optimization of in vitro selection for nitrate reductase-deficient mutants by screening for chlorate resistance. Selection was carried out on haploid mesophyll protoplast-derived cell cultures of Nicotiana plumbaginifolia. It is demonstrated that revised selection results in high variant detectability and increased variant confirmability in comparison with the hitherto used selection scheme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289289
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