ISSN:
1432-2242
Keywords:
RAPDs
;
Experimental design
;
Fractional factorial
;
Chrysanthemum
;
PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Many conditions of the RAPD reaction procedure may influence the result. This paper presents rapid detection of influential factors with a fractional factorial experiment. A more extensive study of these factors is also presented. Polymerase brand, thermal cycler brand, annealing temperature, and primer, are important factors in obtaining good DNA yields and optimal fragment patterns. Each primer has its optimal annealing temperature, and this is not correlated with the GC content of the primer. Optimal species-primer combinations have to be found by trial and error.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00211058
