Electronic Resource
Springer
Archives of microbiology
148 (1987), S. 107-114
ISSN:
1432-072X
Keywords:
Autotrophy
;
Clostridia
;
Clostridium aceticum
;
Plasmids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A plasmid screening was performed on 150 strains out of 75 clostridial species using a modification of the alkaline-lysis procedure. In 26 strains representing 21 species one or more plasmid bands were detected ranging in size from 3 to more than 100 kilobase pairs. Clostridium aceticum proved to contain a single small plasmid (pCA1) of 5.4 kbp as revealed by restriction analysis and electron microscopy. A physical map of pCA1 has been constructed. Spontaneous mutants of C. aceticum defective in autotrophic growth have been isolated. No direct correlation between plasmid content and autotrophy could be found.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425357
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