ISSN:
1432-1912
Keywords:
Histamine
;
Histamine Methyltransferase
;
Gastric Mucosa
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary High histamine concentrations and histamine methyl transferase activity were demonstrated in the gastric mucosa of man, dog, pig and cow. Modified methods for the determination of histamine and histamine methyltransferase were developed. Histamine was identified by its fluorescence spectrum, by thin-layer chromatography in 8 different solvent systems and by bioassay. Histamine methyltransferase from pig antral mucosa was purified 6-fold by ultra-centrifugation and fractional precipitation by ammonium sulfate. ItsK m for histamine as substrate was 2.3×10−5 M, for S-adenosylmethionine 4.3×10−5 M, the pH-optimum was found to be pH 7.4. Nicotinamide in concentrations up to 1×10−2 M had no effect on the activity of the enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00997278
