ISSN:
1432-2013
Keywords:
Microfluorescence photometry
;
Microreflectance photometry
;
Tissue fluorescence
;
NADH-fluorescence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The construction of a microscope photometer using prefabricated elements is described. To illuminate the tissue, a Leitz Ultropac is applied. To enlarge the wavelength range, its illuminating glass lens is replaced by an Acryl glass zonal lens. Two separate light channels with separate lamps, monochromators and photomultipliers allow the measurement of fluorescence excitation and emission spectra as well as of reflection spectra. By chopping the light, light pulses and dark currents are measured 8.33 times a second. By an integration circuit the signal-to-noise ratio for small signals is improved. The instrument allows us to detect the increase of 4 ng/ml NADH (solution of pH 7.38) in an area of 0.2 mm2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00588698
