ISSN:
1432-1041
Keywords:
Conjugate-cleavage
;
drug metabolism
;
gas chromatography
;
phenobarbital
;
urinary excretion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Medicine
Notes:
Summary A method for the quantitative determination of phenobarbital and free and conjugatedp-hydroxyphenobarbital in urine samples is described. The method includes initial extraction, purification on a small chromatographic column and finally determination by gas chromatography. The barbituric acids are methylated by trimethylanilinium hydroxide which serves as a “flash heater” methylating agent. The conjugate ofp-hydroxyphenobarbital, which appears to be a glucuronide, is hydrolysed with hydrochloric acid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00614013