ISSN:
1432-1351
Keywords:
Area postrema
;
Rabbit
;
Patch clamp
;
Glutamate-receptor
;
GABA-receptor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Using the patch-clamp technique in combination with sliced tissue preparation the membrane properties of newborn rabbit area postrema neurons were investigated. The neurons responded upon depolarization with a fast Na +-current followed by an inactivating and non-inactivating K +-current. GABA-activated currents were investigated resulting in a large Cl--conductance, indicating the expression of GABAA-receptors. The expression of glutamate receptor mRNA was studied by in situ hybridization and electrophysiological measurements of these receptors by means of the patch-clamp technique. As a main result it was found that ionotropic glutamate receptors in the area postrema are composed of “flop” variants of the GluA-, GluB- and GluC-subunits.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00225825
