Electronic Resource
Springer
Molecular genetics and genomics
188 (1982), S. 121-127
ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary It is shown that mutants of Saccharomyces cerevisiae able to efficiently utilise exogenous dTMP can also utilise exogenous dAMP. Under extracellular conditions permissive for dTMP uptake label stemming from offered [8-3H]dAMP is incorporated preferentially into alkali-resistant, high molecular weight material (putative DNA): only about 30% of high molecular weight cell-bound dAMP label was found to be sensitive towards mild alkali hydrolysis. This putative RNA label can be minimised to practically zero when ≧mM Ade is employed in a dAMP labelling assay. Exogenous dAMP at ≫10 μM was found to be cytostatic similarly to ≫μM dTMP and similarly to inhibit effectively import of exogenous Pi. We conclude from our results that there exists a yeast cytoplasmic membrane permease able to import dAMP. A model of this hypothetical permease system is presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333005
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