ISSN:
1434-4475
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The purification of anti-BPO-antibodies and anti-BPO-IgG resp. by means of affinity chromatography is being described. The immunosorbent was prepared by direct covalent coupling of BPO groups to AH-Sepharose 4B beads. The antibodies were linked to the immunosorbent in a batch and eluted by stepwise chromatography. The hapten density of the gel was very high (3.6×10−5 mole BPO/1 g Sepharose 4B) as could be demonstrated by application of14C-penicillin-potassium. Good yields of highly purified anti-PBO-antibodies were obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00900901