ISSN:
1615-6102
Keywords:
Amiprophos-methyl
;
Microtubule organizing center
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A one hour exposure to 3 μM amiprophos-methyl (APM) depolymerizes all MT arrays in cells from higher plant suspension cultures. On removal of APM, MT repolymerization sites are detected using immunofluorescent staining. During interphase, Mt arrays return uniformly dispersed across the cell cortex with transverse arrays in elongated cells and random arrays in isodiametric cells. During cell division, MT arrays return as follows: Prophase-MT arrays return in association with the nuclear envelope. Metaphase-MTs return associated with chromosomes. Teleophase-MTs return in apparent association with the reforming nuclear envelope and as aberrant phragmoplasts. MTOCs in higher plant cells may be membrane associated at many stages in the cell cycle. Isolated, condensed chromosomes are capable of nucleating MTs, which can attain small, spindle-like configurations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01320279
