ISSN:
1612-1112
Keywords:
Thin-layer chromatography
;
Porphyrin
;
Metalloporphyrins
;
Hematoporphyrin IX
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The thin-layer chromatographic mobility of hematoporphyrin IX (Hp) and its metal complexes on octyl (C8)- or octadecyl (C18)-bonded silica gel plate is described. The mobility order for those compounds is regular on both kinds of plate with various compositions of methanol-phosphate buffer mixtures as the developing solvents; thus, Cu-complex 〈 Ni-complex 〈 Hp (free acid) 〈 Zn-complex. The combination of a C18-plate with an 85∶15 (vol/vol) mixture of methanol and phosphate buffer (pH 3) is recommended for the successful separation of these four compounds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02310537
