Electronic Resource
[S.l.]
:
American Institute of Physics (AIP)
Review of Scientific Instruments
71 (2000), S. 2201-2205
ISSN:
1089-7623
Source:
AIP Digital Archive
Topics:
Physics
,
Electrical Engineering, Measurement and Control Technology
Notes:
Two different highly sensitive and fast but low-cost instruments for fluorescence measurements on nanotiter plates or other high density sample arrays are presented. Both instruments use 635 nm diode lasers for the detection of Cy5 fluorescence. In the first device all cavities of the nanotiter plate are illuminated simultaneously and the fluorescence is detected spatially resolved by a charge-coupled device camera within a few seconds. The second system uses an on-chip microscanner for the sequential illumination of the samples and the fluorescence is detected by a simple photomultiplier tube. Both instruments have originally been developed for environmental analysis by immunochemical labeling but they can also be used for other medical and biological purposes where analyte concentrations have to be determined. © 2000 American Institute of Physics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1063/1.1150606
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