ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Rapid recovery of cell sheets is considered important to maintain the biological functionand viability of recovered cell sheets. To accelerate required culture substrate hydrophilic/hydrophobic structural changes in response to culture temperature alteration, poly(2-hydroxyethylmethacrylate) (PHEMA) and poly(N-isopropylacrylamide) (PIPAAm) were grafted successivelyonto tissue culture polystyrene (TCPS) dishes by electron beam irradiation. Analysis by attenuatedtotal reflection-Fourier transform infrared revealed that PHEMA and PIPAAm were successfullygrafted to surfaces of TCPS dish. PIPAAm-PHEMA-grafted TCPS (PIPAAm-PHEMA-TCPS)dishes were compared with PIPAAm-grafted TCPS dishes for cell sheet detachment experiments.Approximately 75 min was required to completely detach cell sheets from PIPAAm-TCPS dish,compared to only 13 min to detach cell sheets from PIPAAm-PHEMA-TCPS dish, which issuccessively grafted with PHEMA and PIPAAm. PHEMA is a well-known as a high hygroscopicpolymer. In the case of PIPAAm-PHEMA-TCPS dish, PHEMA layer acted as a water pool toaccelerate the hydration of PIPAAm layer due to the effective and simultaneous water supply toPIPAAm layer, resulting in rapid hydration of grafted PIPAAm molecules and detachment of cellsheet compare to PIPAAm-TCPS dish without PHEMA layer
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.245.pdf
