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  • 1
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 197-200 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this study, we prepared polystyrene (PS) nanofibers as hepatocytes culture substratesby electrospinning method and subsequently coated with specific ligand (poly(N-[removed info]-vinylbenzyl-[removed info]-β-D-galactopyranosyl-(1[removed info]4)-D-gluconamide)(PVLA) for hepatocytes attachment. Rat hepatocytes’behavior on the PVLA-coated and non-coated PS nanofibrous matrices have been investigated.Electrospun PS fiber structures revealed randomly aligned fibers with average diameter of 500 nm.Fabricated PS nanofibers had no bonding points like cotton fibers. Analyses by ATR/FTIR andESCA revealed that PVLA was successfully coated to the surfaces of PS nanofibers. Morehepatocytes were attached on the surface of PS nanofibers coated with PVLA than that on noncoatedPS nanofibers. PS nanofibrous matrix could incorporate many cells into the interior of thematrix probably due to the suitable pore size. Cell viabilities cultured on PVLA-coated PSnanofibrous mats were maintained for 2 weeks, while it was decreased rapidly on PVLA-coated PSdishes. High hepatic function especially albumin secretion was maintained for 2 weeks onnanofibrous mats but rapidly decreased on flat PS dishes. These results indicate that nanofibrousstructure enabled spheroid-like culture results in providing cell-cell communication and subsequentlong-term maintenance of specific cell function
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 201-204 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrousmatrix containing gelatin was fabricated by electrospinning method. The average diameter ofelectrospun PHBV/Gelatin (1:1) nanofibers was 600 nm determined by FE-SEM. ATR-FTIR andESCA measurements were used to confirm the presence of gelatin in PHBV/Gelatin nanofibers.Human fibroblasts' behavior on PHBV/Gelatin nanofibrous matrix has been investigated.Fibroblasts were well attached on the surface of control PHBV and PHBV/Gelatin nanofibers.Initial cell attachment on PHBV/Gelatin nanofibers was higher than that of control PHBVnanofibers. Gelatin has many RGD moiety that mediate cell attachment. From this reason, initialcell attachment increased on the surface of PHBV/Gelatin nanofibers. From the results, coelectrospinningof PHBV and gelatin is a promising method for tissue engineering scaffold
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 225-228 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Poly(γ-glutamic acid)(γ-PGA) based nanofiber sheets were prepared by usingelectrospinning technique to evaluate the ability of the prevention of postoperative tissue adhesion.The anti-adhesion membranes were prepared from poly(γ-glutamic acid) and PLGA with differentcompositions by electrospinning. Also nonsteroidal anti-inflammatory drug (ibuprofen) wasincorporated during fabrication of nanofibers. Various electrospun nanofibers were characterized bythe measurements of microstructure (surface morphology and fiber diameter by SEM), ATR-FTIR,water contact angle and in vivo animal study using Sprague Dawley rat model. The averagediameter of nanofibers electrospun from trifluoroacetic acid (TFA) solution ranged from 300 nm to900 nm, approximately. From in vivo animal study, it was observed that ibuprofen-incorporated γ-PGA nanofiber sheet was significantly effective in preventing tissue adhesion and inducing woundhealing, probably due to the appropriate hydrophilicity of γ-PGA preventing shrinkage of the sheetand appropriate barrier property, while PLGA nanofibrous mat was dramatically contracted in invivo due to its high hydrophobicity resulted in insufficient coverage of wound
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Rapid recovery of cell sheets is considered important to maintain the biological functionand viability of recovered cell sheets. To accelerate required culture substrate hydrophilic/hydrophobic structural changes in response to culture temperature alteration, poly(2-hydroxyethylmethacrylate) (PHEMA) and poly(N-isopropylacrylamide) (PIPAAm) were grafted successivelyonto tissue culture polystyrene (TCPS) dishes by electron beam irradiation. Analysis by attenuatedtotal reflection-Fourier transform infrared revealed that PHEMA and PIPAAm were successfullygrafted to surfaces of TCPS dish. PIPAAm-PHEMA-grafted TCPS (PIPAAm-PHEMA-TCPS)dishes were compared with PIPAAm-grafted TCPS dishes for cell sheet detachment experiments.Approximately 75 min was required to completely detach cell sheets from PIPAAm-TCPS dish,compared to only 13 min to detach cell sheets from PIPAAm-PHEMA-TCPS dish, which issuccessively grafted with PHEMA and PIPAAm. PHEMA is a well-known as a high hygroscopicpolymer. In the case of PIPAAm-PHEMA-TCPS dish, PHEMA layer acted as a water pool toaccelerate the hydration of PIPAAm layer due to the effective and simultaneous water supply toPIPAAm layer, resulting in rapid hydration of grafted PIPAAm molecules and detachment of cellsheet compare to PIPAAm-TCPS dish without PHEMA layer
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 249-252 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: PHBV ultrafine fibers were fabricated by electrospinning process. Electrospun PHBVfiber structures revealed randomly aligned fibers with average diameter of 400 nm. PIPAAm wasgrafted on the surface of PHBV nanofibrous mat by electron beam irradiation. PIPAAm-graftedPHBV mats were determined by ATR-FTIR and ESCA. Water contact angles were determined by asessile drop method at 20 and 37[removed info]. To examine the tissue compatibility, human fibroblasts wereevenly seeded onto PIPAAm-grafted PHBV mat and cast film, ungrafted PHBV mat and film.Attached and spread fibroblasts on nanofibrous mat were proliferated more rapidly than that of flatfilm surface. Initial cell attachment on PIPAAm-grafted surfaces was higher than ungraftedsurfaces. The surface property changed to hydrophilic by PIPAAm graft, which increased initial cellattachment. Detachment of single cells from PIPAAm-grafted PHBV matrixes was measured bylow temperature treatment after incubation at 37[removed info]. Cultured cells were rapidly detached fromPIPAAm-grafted PHBV mat compared with film. With porous mats, the water molecules easilyreach to grafted PIPAAm from underneath and peripheral to the attached cells, resulting in rapidhydration of grafted PIPAAm molecules and detachment of the cells
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 1807-1810 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Blends prepared from poly(ethylene 2,6-naphthalate) (PEN) and poly(butylene 2,6-naphthalate) (PBN) show only partial miscibility judged from their glass transition temperatures. Two distinct mechanical behaviors are observed: brittle for the blends 〈 20 wt% of PBN, while ductile 〉 20 wt% of PBN. The experimental modulus and strength values of the blends are within the predicted values according to Kleiner and Paul models, respectively. This means that PEN/PBN blends are somewhat compatible based on their tensile properties. Especially for 20 wt% of PBN blend, the high modulus and strength are observed. The viscosity of the blend is high, which may imply a somewhat entangled morphology in the amorphous state.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 703-708 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A reactor with surface renewal, originally designed for poly(ethylene terephthalate) (PET) polymerization, was applied for poly(butylene terephthalate) (PBT) polymerization. A comprehensive model including side reactions was developed and compared with the experimental results. The diffusivity of butanediol (BD) in PBT melt was measured separately by desorption experiments (Db - 1.08 × 106 exp(-32600 / RT) (m2/min)). Optimum operating temperature for PBT polymerization was found to be around 250°C in order to avoid degradation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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