Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
European journal of neuroscience
5 (1993), S. 0
ISSN:
1460-9568
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
During early postnatal development of the kitten visual cortex the ectoenzyme 5′-nucleotidase undergoes a characteristic redistribution. Until about postnatal week 6 it is essentially confined to synaptic contacts in input layer IV and its expression is related to the use-dependent segregation of thalamic afferents into ocular dominance columns. Subsequently, 5′-nucleotidase becomes distributed uniformly throughout all layers and is then associated selectively with glial cells. Here we describe an age-dependent alteration in the expression of a carbohydrate epitope of 5′-nucleotidase which correlates with the developmental change of the enzyme's localization. We have isolated 5′-nucleotidase from the occipital cortex of kittens of varying age and from adult cats and investigated by immunoblotting the association of the HNK-1 carbohydrate epitope with the protein. 5′-Nucleotidase carries the HNK-1 epitope in kittens of 3–9 weeks but the epitope is absent from 12-week-old kittens or adult cats. Thus, the appearance of the HNK-1 epitope correlates with the transient localization of the enzyme at synapses. The HNK-1 carrying 5′-nucleotidase may be involved in synaptogenesis and use-dependent modifications of synaptic connections.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1460-9568.1993.tb00209.x
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