ISSN:
1365-2826
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
GAWK was isolated from human pituitary glands and presumed to be a processing product of human Chromogranin B. In the present studies, we investigated effects of phorbol ester (TPA), forskolin and Ca2+-ionophore (A23187) on GAWK secretion using a human medullary thyroid carcinoma-derived cell line (TT-cell). A dose-responsive stimulation of GAWK-like immunoreactivity (LI) secretion was found in the presence of TPA as well as forskolin. A23187 caused an increase of GAWK-LI secretion and addition of TPA together with A23187 revealed an additive effect on the GAWK-LI secretion. These findings indicate that GAWK-LI is a secretory peptide in the TT-cells and suggest that A and C kinase, and possibly Ca2+-calmodulin transduction systems are involved in the GAWK-LI secretion by the TT-cells. TT-cells may provide a good model for the investigation of regulation of Chromogranin secretion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2826.1990.tb00440.x