ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
—1,2-Diacyl-, 1-alk-1′-enyl-2-acyl-and 1-alkyl-2-acyl-sn-glycero-3-phosphorylcholine, specifically labelled with different fatty acids at the 2 position, were prepared enzymically using the acyltransferase system of rabbit sarcoplasmic reticulum. The substrates were submitted to hydrolysis by mitochondrial phospholipase A2 (phosphatide acyl-hydrolase, EC 3.1.1.4) obtained from normal and from rat brain afflicted with EAE. In the acute stage of the disease an increase of approximately 25 per cent in phospholipase A 2 activity could be observed in comparison to that from the control animals for all investigated substrates. Phospholipase A2 obtained from normal rat brains and from those afflicted with EAE had a higher affinity for 1,2-diacyl-sn-glycero-3-phosphorylcholine when compared to the corresponding alkyl acyl- and alkenyl acyl-analogues. Choline plasmalogen was cleaved more slowly than the corresponding alkyl acyl derivative. The enzyme activity returned to the control level in the recovery stage of the demyelinating disease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1974.tb04399.x