ISSN:
1365-3040
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Messenger RNA from salt-sensitive and salt-tolerant plants Triticum aestivum. Beta vulgaris, Pisum sativum, Chenopodium album and Atriplex nummularia was translated in vitro in a wheatgerm translation system. The optimal monovalent and divalent ion concentrations for translation were independent of the salt tolerance of the plants from which the m-RNAs were derived. Translation was optimal in 100 120 mol m−3 potassium acetate and 1.5–2.0 mol m−3 Mg2+. Substitution of Na+ for K+, or of Cl− for acetate, was inhibitory. The pattern of polypeptides synthesized from cytoplasmic m-RNAs of salt-sensitive and salt-tolerant plants remained constant in all the conditions examined. The effects of adding the ‘compatible' organic solutes glycine-betaine and mannitol were examined in the wheat-germ system primed with RNA from the leaves of Triticum aestivum or Beta vulgaris. The rate of translation, the optimum ionic concentrations and the distribution of polypeptide products were maintained in organic solute concentrations of up to 500 mol m−3. Proline above 300 mol m−3 and surcose above 100 mol m−3 did inhibit translation. The results indicate that translation in plants is unlikely in cytoplasmic K+ concentrations exceeding 180 mol m−3, but would proceed in the presence of up to 500 mol m−3 mannitol or glyinebetaine, or of up to 300 mol m−3 proline.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/1365-3040.ep11591845
