ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
A mitogenic component, designated fraction C (Fr C), has been purified from a mutanolysin enzyme digest of Actinomyces cell walls by CM Sephadex C-25 ion-exchange and G-100 gel filtration chromatography. Good mitogenic responses were obtained with Fr C over a broad dose range with peak mitogcnesis seen with 500 μg/culture. Fraction C (mol. wt. = 35,000–40,000) consists of 755 carbohydrate and 23% protein, is non-dialy-sable, resistant to heat, lysozyme or protease treatment, and partially sensitive to base, and all milogenic activity is destroyed by cither periodate or acid treatment. Fraction C is a B-cell mitogcn since it induced responses in nude (nu/nu) and nu/+ BALB/c spleen cell cultures and purified splenic B-cell cultures, but did not stimulate purified splenic T-cell cultures. Similar milogenic fractions for B cells have been obtained from cell walls of A naeslundii and from a human isolate of A. viscosus. Good polyclonal IgM synthesis and plaque-forming cell responses to hapten or erythrocytes were obtained in vitro with the purified cell wall fractions derived from all three Actinomyces strains studied. These results indicate that the Actinomyces cell wall possesses a carbohydrate-rich component which activates B cells und may represent a common determinant of this genus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1983.tb00795.x
