ISSN:
1750-3841
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
A membrane filter-disc immunoimmobilization technique was developed for rapid detection of salmonellae in foods. The method involved concentrating bacteria from the selective enrichment culture of a food sample by membrane filtration. The membrane filter, with the entrapped bacteria, was inverted and placed on the surface of a semi-solid selective medium contained in a 100 × 15 mm plastic Petri plate. A paper disc impregnated with Salmonella polyvalent flagellar antiserum was placed on the surface of the semi-solid agar approximately 2.5 cm from the nearest edge of the membrane filter. The plate was incubated at 37° C under high humidity. Motile salmonellae, if present in the sample, grew and migrated in the semi-solid medium. When the moving front of motile salmonellae came into contact with the diffusing flagellar antiserum, an antigen-antibody reaction occurred resulting in the immobilization of salmonellae. The formation of a line of immobilization indicated the presence of salmonellae in the sample. A semi-solid medium containing dulcitol, proteose peptone, brilliant green, and novobiochin as the major functional components was found to be more efficient than semi-solid modifications of Salmonella-Shigella agar and Hektoen enteric agar for the detection of salmonellae in foods by the membrane filter-disc immunoimmobilization procedure. The new method, when applied to the detection of salmonellae in raw meats and poultry, was found to give good correlation with the conventional cultural method.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-2621.1978.tb02516.x
