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Digitale Medien

Antibody production in Japanese eels, Anguilla japonica Temminck & Schlegel (1997)

Hung, H-W ; Lo, C-F ; Tseng, C-C ; [weitere]

Oxford, UK : Blackwell Science Ltd

Journal of fish diseases 20 (1997), S. 0

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Details

ISSN: 1365-2761

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie , Medizin

Notizen: Adult Japanese eels, Anguilla japonica Temminck & Schlegel, (200–250 g, 45–55 cm) were immunized by intramuscular injection with goat IgG. After 5 weeks, eel immunoglobulin (Ig) was purified using affinity chromatography. The purified eel Ig was used to immunize rabbits to produce anti-eel Ig antibody. The highest antibody ELISA value in eels was reached 3 weeks after initial immunization with goat IgG, and then gradually decreased. The antibody could still be detected at 140 days post-immunization. The optimal temperature for antibody production was 30°C. Freund’s complete adjuvant and secondary immunization both increased antibody production in eels.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1046/j.1365-2761.1997.00290.x

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