ISSN:
0935-6304
Keywords:
Gas chromatography
;
Capillary columns
;
Steroid analysis
;
Sample pretreatment
;
Sample introduction
;
Separation speed
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Quantitative analysis of steroids and their metabolites in urine samples calls for increased speed of sample clean-up, of the derivatization procedure, and of separation. A fast procedure for sample pretreatment, which can be performed within 8 hours, is introduced and evaluated. It is shown that use of fast pretreatment in combination with narrow bore columns, which are compatible with existing instrumentation, can considerably increase, laboratory throughput. The effect of different sample introduction techniques (e.g. splitless, on-column, and moving needle) on column efficiency and resolution is demonstrated and discussed.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240070713
