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Digitale Medien

Direct peptide mapping of sickle-cell hemoglobin using electrospray mass spectrometry (1993)

Sakairi, Minoru

New York, NY : Wiley-Blackwell

Rapid Communications in Mass Spectrometry 7 (1993), S. 1108-1112

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Details

ISSN: 0951-4198

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Physik

Notizen: Direct peptide mapping of sickle-cell hemoglobin (Hb S) using electrospray mass spectrometry is demonstrated to be a rapid, sensitive and useful technique for providing information concerning the location of variation. By fully comparing electrospray mass spectra of digests of sickle-cell and normal hemoglobins, using L-l-tosylamide-2-phenylethyl chloromeihyl ketone (TPCK)-treated trypsin, the point mutation of B1 fragment of β-chain in Hb S can be confirmed, taking into consideration that all the other fragments are completely identical in the two proteins.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/rcm.1290071210

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