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Digitale Medien

Reversed phase high performance liquid chromatography of glycopeptides (1989)

Wang, Tsonghui ; Chen, Tsefang ; Barofsky, Douglas F.

New York, NY : Wiley-Blackwell

Biomedical Chromatography 3 (1989), S. 241-245

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Details

ISSN: 0269-3879

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: A practical procedure for isolating and purifying glycopeptides is described, viz. enzymatic hydrolysis - gel permeation chromatography - ion exchange chromatography - reversed phase HPLC. Using this procedure 28 glycopeptides from hen ovalbumin have been isolated some of which hitherto have not been identified. Water was a suitable mobile phase for preparing pure glycopeptides, and control of column temperature was important for good separations and reproducible retention times. Structural confirmation was by fast atom bombardment mass spectrometry.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bmc.1130030603

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