ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A new procedure for the simultaneous measurement of prostaglandins E2 and F2α in human urine is described. Quantification was achieved by gas chromatography mass spectrometry with selected ion monitoring and deuterium labeled internal standards. The levels of measured prostaglandins ranged from 25 pg ml-1 of urine (PGE2) to 2500 pg ml-1 (PGF2α). The interassay coefficient of variation, determined by replicate analysis of four identical samples, was 1.9% for PGE2 and 0.8% for PGF2α. The procedure takes a fraction of the time needed with published methods and can be conducted with as little as one-tenth of the daily urinary output.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200061005
