ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Conditions were established for analyzing as little as 5 pmol of an underivatized peptide delivered in a glycerol sample matrix as a thin film onto a gold-plated copper sample stage and then bombarded with xenon fast atoms. Calibration of the fast atom bombardment high field mass spectrometer and data system was achieved using cesium iodide/glycerol as a reference. Calibration at several accelerating potentials permitted a mass range from 393 to 5941 u. Several factors were examined that contribute to the quality of the mass spectrum: components within the glycerol such as other peptides, alkali salts, acid and reducing agents; the nature of the fast atom gas; concentration of the peptide delivered to the sample stage; and the effect of the sample stage and sample matrix on sensitivity.
Additional Material:
13 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200100504
