ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Platelet activating factor has recently been structurally described as the phospholipid 1-O-alkyl-2-O-acetyl-sn-glycerophosphocholine. It has been reported to be a putative chemical mediator of immunological reactions as well as an antihypertensive agent. A method to quantitate 1-O-alkyl-2-O-acetyl-sn-glycerophosphocholine using deuterium labeled 1-O-alkyl-2-O-acetyl-sn-glycerophosphocholine as an internal standard is described. When 60 ng of the2H3 labeled compound is employed, quantities as low as 10 ng can be measured. The standard curve for this quantitative analysis typically had a correlation coefficient of 0.999 even when the signal saturation effect of FAB was apparent. Thus quantitative analysis using the FAB ionization technique is feasible when one employs the stable isotope internal standard method.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200110109
