ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Extracts derived from rat liver and Phaseolus leaves are shown, by collision-induced dissociation of [MH]+ ions generated by fast atom bombardment mass spectrometry, to contain cytidine 3′,5′-cyclic monophosphate and guanosine 3′,5′-cyclic monophosphate respectively, and not the 2′,3′-cyclic isomers. Interference peaks, expected to be common to all mass-analysed ion kinetic energy spectra of ions generated by the fast atom bombardment process from glycerol-based matrices are identified. It is shown that unequivocal identification of cytidine 3′,5′-cyclic monophosphate can be made at the microgram level. Attempts to derive a quantitative procedure based on using different cyclic nucleotides as internal standards were unsuccessful due to the poor solubility of these compounds in the matrix system.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200110709
