ISSN:
0030-4921
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The two C-4 protons of reduced nicotinamide adenine dinucleotide (NADH) produce an AB NMR spectrum at 100 MHz as well as at 220 MHz. This observation allows an upper limit of 50 sec-1 to be placed on the mean rate of interconversion of the two folded forms of NADH invoked to account for the magnetic non-equivalence of the C-4 protons. The interpretation of non-equivalence of the C-4 protons in terms of the various equilibria among folded and unfolded forms of NADH and its possible significance in the mechanism of action of dehydrogenase enzymes is discussed. It is suggested that one folded form of NADH is strongly favored thermodynamically over the other and that the resulting magnetic non-equivalence of the C-4 protons is of doubtful significance in explaining the stereospecificity of dehydrogenase enzymes toward the nicotinamide ring.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1270010404
