ISSN:
0030-493X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An unambiguous differentiation between leucine and isoleucine residues in peptides is possible with unimolecular metastable ion (MI) and collisional activation (CA) spectra. The immonium ion \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm [Y}\!\mathop {\rm N}\limits^{\rm + } {\rm H = CHC}_{\rm 4} {\rm H}_{\rm 9} {\rm ]} $\end{document}, which is present in the normal mass spectra of all leucine or isoleucine containing peptides, yields MI spectra with which this differentiation can be made with high sensitivity. The MI and CA spectra of other peptide fragments are also applicable in particular cases. MI and CA spectra can also be used to obtain information on the amino acide sequences of individual ions. In general more extensive sequece information is available from CA than from MI spectra. This information appears to be particularly promising for the sequencing of oligopeptide mixtures or of ions produced by field or chemical ionization.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/oms.1210080116
