ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Search strategies for finding spot differences among multiple two-dimensional (2-D) polyacrylamide electrophoresis gels are discussed in the context of the GELLAB spot data base management system. A 2-D gel experiment should have a well-defined biological experimental and preparation protocol reflecting the hypotheses of the problem. So too should the analysis of its corresponding 2-D gel computer spot data base have a protocol. This protocol is heavily influenced by the nature of the biological experiment as well as 2-D gel preparation considerations including the realities of artifactual and systematic noise. It is further influenced by constraints due to computational considerations. The search strategy is that part of the analysis protocol in which an experimenter iteratively defines tests to find significant spot differences. One goal of designing a well thought out search protocol is to reduce the number of search iterations required. Aspects of some requirements and constraints for useful search strategies are discussed.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040110