ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The native protein patterns of human serum and unconcentrated cerebrospinal fluid, amniotic fluid, urine, sweat and tears have been detected by silver staining following two-dimensional electrophoresis in the absence of protein denaturants. Over 100 proteins were detected in both urine (80 μl) and sweat (40 μl), thereby demonstrating the high sensitivity of the approach. However, optimal sensitivity was only achieved following pre-equilibration of the gels in sodium dodecyl sulphate. The implications and possible nature of stain enhancement are discussed.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150050612
