ISSN:
0886-9383
Keywords:
ATP hydrolysis
;
Regression analysis
;
Model discrimination
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Data of the hydrolysis of adenosine 5′-triphosphate have been re-evaluated using a computer program that numerically integrates the differential rate equations within a routine that optimizes rate coefficients given a suitable model and concentration versus time data.The model is tested by calculation of Hamilton R-values, the Fisher F-statistic, a sensitivity analysis, the standard errors on the rate coefficients and by constructing contour maps of the objective function versus two rate coefficients.An optimization using only phosphate concentration data cannot distinguish between a model in which adenosine 5′-monophosphate is formed predominantly directly with a molecule of pyrophosphate, and one in which it is formed via adenosine 5′-diphosphate. A more accurate set of rate coefficients is calculated from existing data and the relative importance of the two paths determined.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cem.1180030405
