ISSN:
1075-4261
Keywords:
DNA
;
silica
;
binding
;
Raman spectroscopy
;
infrared
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Physics
Notes:
We studied by infrared and Raman spectroscopy the interaction of calf thymus DNA with various types of silica particles, ranging in size from 7 nm to 60 μm. Preliminary experiments with different samples showed that substantial variations can take place in the 1000-1100 cm-1 region of the attenuated total reflection (ATR) spectrum of silica, where a strong band due to a stretching vibration of the Si-O groups occurs. The position and intensity of this band were found to be dependent on several parameters, such as the size distribution of the solid particles, their proximity to the surface of the ATR crystal, and their degree of packing after sedimentation from the aqueous suspension. Changes observed in the spectra of aqueous solutions of DNA interacting with silica particles are explained by a shift of the main silica band in the mixtures. This interpretation differs from that of a previous study, where important intensity variations of the DNA bands at 1086 and 1053 cm-1 were explained by the formation of hydrogen bonds between the silanol groups of silica and the phosphate groups of DNA. Raman spectra of aqueous solutions of DNA mixed with fumed quartz particles of an average size of 0.007 μm showed but a minor change in intensity (ca. 5%) of the DNA symmetric phosphate band, which supports the conclusion reached in our infrared study. © 1997 John Wiley & Sons, Inc. Biospectroscopy 3: 299-306, 1997
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
