ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Conformational change of histone IV, induced by phosphate, have been investigated by observing the intrinsic fluorescence of tyrosine residues and circular dichroism (CD). There is a fast conformational change upon the addition of phosphate, followed by a slow process with time constants in the range of minutes to hours depending upon both the phosphate and histone concentrations. The CD results indicate α-helix formation in the fast process, and β-sheet formation in the slow one, although other secondary and tertiary structural changes also may occur. The histone concentration dependence of the fast process is consistent with dimerization. Divalent phosphate is about ten times more effective than monovalent phosphate in inducing conformational changes. All of the changes are reversible.
Additional Material:
17 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1972.360110206
