ISSN:
0006-3525
Schlagwort(e):
Chemistry
;
Polymer and Materials Science
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
We report the predicted combining site structure of the monoclonal antibody fragment. NC10.14, which is specific for the superpotent sweetener, N-(p-cyanophenyl-N′-(diphenylmethyl)guanidine acetic acid, using computer-aided molecular modeling and experimental methods, such as fluorescence spectroscopy and circular dichroism. This is the first computer-aided modeling study on a λ-chain antibody fragment. We have also identified the amino acids that are involved in ligand binding. Aromatic residues. L:91(W), L:96(W), and H:100G(Y) are predicted to make van der Waals contacts with the p-cyanophenyl moiety of the ligand. Residue H:56(K) is predicted to provide a counterion for the acetic acid moiety, and H:50(E) provides the negatively charged potential for interaction with the positive guanidinium group. We also make a comparison of the binding site architecture of NC10.14 with that of a related monoclonal antibody fragment NC6.8. © 1996 John Wiley & Sons, Inc.
Zusätzliches Material:
7 Ill.
Materialart:
Digitale Medien
