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A method of automatic pH control of a bicarbonate-CO2 buffer system for the submerged culture of hamster kidney cells (1964)

Telling, R. C. ; Stone, C. J.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 6 (1964), S. 147-158

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The equipment and operational techniques are described which were found suitable to control pH in the range 6.8-7.8 pH, within ±0.03 pH units of the desired value, at cell concentrations up to a maximum of 2.5 × 106/ml. The results of batch growth of a suspension strain of the BHK cell (clone 13) under conditions of controlled pH are given and the significance of these results is discussed.

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260060204

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