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Affinity chromatography purification of diphtheria toxin (1974)

Cukor, George ; Readio, Josephine D. ; Kuchler, Robert J.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 16 (1974), S. 925-931

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: NAD was covalently linked to Sepharose-4B using a 6 carbon spacer. Sterile, dialyzed spent culture medium containing 100 Lf/ml of diphtheria toxin or material concentrated by (NH4)2SO4 precipitation containing 1500 Lf/ml, was chromatographed on a column of NAD-Sepharose. Ultraviolet absorbing material which did not flocculate with diphtheria antitoxin was eluted with 0.02M phosphate buffer. When the elation buffer was changed to one containing 0.5M NaCl, purified toxin was eluted off the column.

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260160706

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