ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The effect of chaotropic salts on the dissociation of ribonucleic acid from yeast nucleoprotein complex was studied. The effectiveness of various salts on the dissociation of ribonucleic acid followed the chaotropic series; i.e., Cl3CCOONa = NaClO4 〉 NaBr 〉 NaCl. Treatment of the nucleoprotein complex with 0.5M Cl3CCOONa or NaClO4 resulted in RNA removal of about 80%, whereas NaCl and NaBr removed only about 10 and 25%, respectively. Based on the results presented, a simple and novel method for industrial-scale preparation of single-cell proteins with low levels of nucleic acid is proposed.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260250312
