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A Process for large-scale isolation of β-galactosidase from E. coli in an aqueous two-phase system (1983)

Veide, Andres ; Smeds, Anna-Lisa ; Enfors, Sven-Olof

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 25 (1983), S. 1789-1800

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A method suitable for large-scale isolation of β-galactosidase from a suspension of disintegrated E. coli cells has been developed. In an aqueous two-phase system consiting of PEG 6000 and potassium phosphate, all cell debris and the major part of the proteins and nucleic acids were partitioned to the denser salt phase. Seventy-five percent of the β-galactosidase was recovered in the lighter PEG phase, giving a purification ratio of about 12.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260250709

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